ДСТУ EN ISO 22964:2019 Микробиология пищевой цепи. Горизонтальный метод определения Enterobacter sakazakii (EN ISO 22964:2017, IDT; ISO 22964:2017, IDT)

ДСТУ EN ISO 22964:2019
(EN ISO 22964:2017, IDT;
ISO 22964:2017, IDT)

Мікробіологія харчового ланцюга.
Горизонтальний метод визначення Enterobacter sakazakii

 
   
 
 
     
Не є офіційним виданням.
Офіційне видання розповсюджує національний орган стандартизації
(ДП «УкрНДНЦ» http://uas.gov.ua)

Contents

Foreword

Introduction

1 Scope

2 Normative references

3 Terms and definitions

4 Abbreviated terms

5 Principle

5.1 Non-selective pre-enrichment in BPW

5.2 Enrichment in a selective medium (CSB)

5.3 Plating out and identification on chromogenic agar (CCI agar)

5.4 Confirmation

6 Culture media and reagents

7 Equipment and consumables

7.10 Pestle and mortar

8 Sampling

9 Preparation of test sample

10 Procedure (as shown in Annex A)

10.1 Test portion

10.2 Pre-enrichment

10.3 Enrichment

10.4 Isolation of presumptive Cronobacter spp

10.5 Confirmation

10.6 Interpretation of biochemical results

11 Expression of results

12 Performance characteristics of the method

12.1 Interlaboratory study

12.2 Sensitivity

12.3 Specificity

12.4 LOD₅₀

13 Test report

Annex A (normative) Diagram of the test procedure

Annex B (normative) Composition, preparation and performance testing of culture media and reagents

Annex C (informative) Distinction of Cronobacter spp. from other genera

Annex D (informative) Method validation studies and performance characteristics

Bibliography