ДСТУ EN ISO 11290-1:2022 Микробиология пищевой цепи. Горизонтальный метод обнаружения и подсчета Listeria monocytogenes и Listeria spp. Часть 1. Метод обнаружения (EN ISO 11290-1:2017, IDT; ISO 11290-1:2017,...



ДСТУ EN ISO 11290-1:2022
(EN ISO 11290-1:2017, IDT; ISO 11290-1:2017, IDT)

Мікробіологія харчового ланцюга. Горизонтальний метод виявлення та
підрахунку Listeria monocytogenes і Listeria spp. Частина 1. Метод виявлення

 

 
   
 
Не є офіційним виданням.
Офіційне видання розповсюджує національний орган стандартизації
(ДП «УкрНДНЦ» http://uas.gov.ua)

Contents

Foreword

Introduction

1 Scope

2 Normative references

3 Terms and definitions

4 Principle

4.1 General

4.2 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half-Fraser broth)

4.3 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)

4.4 Plating out and identification

4.5 Confirmation

5 Culture media and reagents

6 Equipment and consumables

7 Sampling

8 Preparation of test sample

9 Procedure

9.1 Test portion and initial suspension

9.2 Primary enrichment

9.3 Secondary enrichment

9.4 Plating out and identification

9.5 Confirmation of Listeria monocytogenes or Listeria spp

9.6 Interpretation of morphological and physiological properties and of the biochemical reactions

9.7 Additional characterization of isolated strains (optional)

10 Expression of results

11 Performance characteristics of the method

11.1 Method validation studies

11.2 Sensitivity

11.3 Specificity

11.4 Level of detection (LOD50)

12 Test report.

13 Quality assurance

Annex A (normative) Diagram of procedure

Annex В (normative) Composition and preparation of culture media and reagents

Annex C (informative) Distinction of Listeria spp. from other genera

Annex D (informative) Reactions for the identification of Listeria species

Annex E (informative) Listeria selective agars

Annex F (informative) Results of the interlaboratory studies for detection of Listeria monocytogenes

Bibliography