ДСТУ ISO 7218:2014 Микробиология пищевых продуктов и кормов для животных. Общие руководства по микробиологическим исследованиям (ISO 7218:2007,ISO 7218:2007/Amd 1:2013, IDT)

ПІДТВЕРДЖУВАЛЬНЕ ПОВІДОМЛЕННЯ

Міністерство економічного розвитку і торгівлі України

Наказ від 30.12.2016 № 1494

 ISO 7218:2007, ISO 7218:2007/Amd 1:2013

Microbiology of food and animal feeding stuffs --
General requirements and guidance for microbiological examinations

прийнято як національний стандарт
методом «підтвердження» за позначенням
 

ДСТУ ISO 7218:2014
(ISO 7218:2007,ISO 7218:2007/Amd 1:2013, IDT)

Мікробіологія харчових продуктів і кормів для тварин.
Загальні настанови щодо мікробіологічних досліджень

З наданням чинності від 2016–01–01

Contents

Foreword

Introduction

1 Scope

2 Normative references

3 Premises

3.1 General

3.2 Safety considerations

3.3 Laboratory design

3.4 Laboratory areas

3.5 Layout and fittings of the premises

3.6 Cleaning and disinfection

4 Staff

4.1 General

4.2 Competence

4.3 Verification of on-going staff competence

4.4 Hygiene

5 Apparatus and equipment

6 Preparation of glassware and other laboratory materials

6.1 Preparation

6.2 Sterilization/decontamination

6.3 Disposable equipment and materials

6.4 Storage of clean glassware and materials

6.5 Management of sterile glassware and materials

6.6 Use of decontamination and disinfection

6.7 Waste management

6.8 Washing

7 Preparation and sterilization of culture media

8 Laboratory samples

8.1 Sampling

8.2 Transport

8.3 Receipt

8.4 Storage

8.5 Test portion

9 Examination

9.1 Hygienic precautions during analysis

9.2 Preparation of initial suspension and dilutions

10 Enumeration

10.1 General

10.2 Enumeration using a solid medium

10.3 Calculation and expression of results obtained with solid media

10.4 Enumeration of yeasts and moulds

10.5 Enumeration using a liquid medium

11 Detection method (qualitative method)

11.1 General

11.2 Principle

11.3 Measurement of uncertainty

12 Confirmatory methods

12.1 General

12.2 Preparation of a pure culture

12.3 Gram’s stain (modified Hucker technique)

12.4 Use of biochemical galleries for identification

12.5 Use of nucleic probes for identification

12.6 Serological methods

13 Test report

14 Validation of microbiological methods

14.1 Validation of reference methods

14.2 Validation of alternative methods

14.3 Validation of in-house methods

15 Quality assurance of results/quality control of performance

15.1 Internal quality control

15.2 Reference strains

15.3 External quality assessment (proficiency testing)

Annex A (informative) Properties of some disinfectants

Annex B (normative) Determination of most probable number (MPN)

Bibliography