ДСТУ EN ISO 11731:2022 Якість води. Виявлення та підрахування Legionella (EN ISO 11731:2017, IDT; ISO 11731:2017, IDT)

ДСТУ EN ISO 11731:2022
(EN ISO 11731:2017, IDT; ISO 11731:2017, IDT)

Якість води. Виявлення та підрахування Legionella

 
   
 
 
     
Не є офіційним виданням.
Офіційне видання розповсюджує національний орган стандартизації
(ДП «УкрНДНЦ» http://uas.gov.ua)

Contents

Foreword

Introduction

1 Scope

2 Normative references

3 Terms and definitions

4 Principle

4.1 General

4.2 Examination

4.3 Confirmation

5 Apparatus and glassware

6 Culture media and reagents

7 Sampling

8 Procedure

8.1 Samples

8.2 Concentration of water samples

8.2.1 General

8.2.2 Membrane filtration and direct placing of the membrane filter on culture media

8.2.3 Membrane filtration followed by a washing procedure

8.3 Sample pre-treatment

8.3.1 Heat treatment

8.3.2 Acid treatment

8.4 Culture

8.4.1 General

8.4.2 Samples with a high concentration of Legionella species and a low concentration of interfering microorganisms

8.4.3 Samples with a low concentration of Legionella species and a low concentration of interfering microorganisms

8.4.4 Samples with a high concentration of interfering microorganisms

8.4.5 Samples with an extremely high concentration of interfering microorganisms

8.4.6 Incubation

8.4.7 Examination of the plates

8.5 Confirmation of presumptive Legionella colonies on culture media: BCYE agar and BCYE-cys agar

9 Expression of results

10 Test report

11 Quality assurance

11.1 General

11.2 Performance testing of Legionella culture media

11.3 Preparing working culture and test suspension for performance testing

Annex A (informative) Legionella species

Annex B (normative) Culture media

Annex C (normative) Diluents

Annex D (normative) Acid solution

Annex E (informative) Scraping or rubbing the bacteria from membrane filters

Annex F (informative) Centrifugation technique

Annex G (informative) Indirect immunofluorescent antibody assay for the identification of Legionella species

Annex H (informative) Performance data

Annex I (informative) Pre-treatment of water related matrices

Annex J (normative) Decision matrix

Bibliography