ДСТУ EN ISO 16014-5:2022 Пластмассы. Определение средней молекулярной массы и распределения молекулярной массы полимеров с помощью эксклюзивной хроматографии. Часть 5. Метод светорассеяния (EN ISO 16014-5:201...



ДСТУ EN ISO 16014-5:2022
(EN ISO 16014-5:2019, IDT; ISO 16014-5:2019, IDT)

Пластмаси. Визначення середньої молекулярної маси та розподілу молекулярної маси полімерів за допомогою ексклюзійної хроматографії. Частина 5. Метод світлорозсіювання

 
   
 
 
     
Не є офіційним виданням.
Офіційне видання розповсюджує національний орган стандартизації
(ДП «УкрНДНЦ» http://uas.gov.ua)

Contents

European foreword

Endorsement notice

Foreword

1 Scope

2 Normative references

3 Terms and definitions

4 Symbols

5 Principle

5.1 SEC

5.2 Light-scattering SEC

6 Reagents

6.1 Eluent

6.2 Reagent for column evaluation

6.3 Calibration standards

6.4 Reagent for flow rate marker

6.5 Additives

7 Apparatus

7.1 General

7.2 Eluent reservoir

7.3 Pumping system

7.4 Injector

7.5 Columns

7.5.1 General

7.5.2 Determination of theoretical plate number

7.5.3 Determination of resolution factor

7.5.4 Determination of asymmetry factor

7.6 Detector

7.6.1 Concentration-sensitive detector

7.6.2 Light-scattering detector

7.7 Tubing

7.8 Temperature control

7.9 Recorder and plotter

7.10 Data-processing system

7.11 Other components

8 Procedure

8.1 Preparation of calibration solutions

8.2 Preparation of a solution for determining the L-point

8.3 Preparation of sample solutions

8.4 Preparation of solutions for column performance evaluation

8.5 Setting up the apparatus

8.6 Operating parameters

8.6.1 Flow rate

8.6.2 Injection masses and injection volumes

8.6.3 Column temperature

8.6.4 Detector sensitivity

8.7 Number of determinations

9 Calibration

9.1 Calibration of concentration-sensitive detector and light-scattering detector

9.1.1 General

9.1.2 Calibration method A

9.1.3 Calibration method B

9.1.4 Calibration method C

9.2 Determination of delay volume

9.3 Normalization of detector sensitivity

9.4 Determination of refractive index increment

10 Data acquisition and processing

10.1 Data acquisition

10.2 Evaluation of data and correction of chromatograms

10.3 Data processing

10.3.1 Baseline determination

10.3.2 Determination of calculation range

10.3.3 Calculation of signal intensity

10.3.4 Calculation of molecular weight

10.3.5 Second virial coefficient, A2

11 Expression of results

11.1 Calibration curve

11.1.1 General

11.1.2 Method A

11.1.3 Method B

11.2 Calculation of average molecular weight

11.3 Differential molecular weight distribution curve

11.4 Cumulative molecular weight distribution curve

12 Precision

13 Test report

13.1 General

13.2 Apparatus and measurement parameters

13.3 Calibration of the system

13.4 Calibration curve

13.5 Results

Annex A (informative) Interlaboratory test

Annex B (informative) Information on light scattering

Annex C (informative) Calibration curve in low molecular weight range

Bibliography