ДСТУ EN 16421:2022 Воздействие материалов на воду для потребления человеком. Усиление микробного роста (EMG) (EN 16421:2014, IDT)



ДСТУ EN 16421:2022
(EN 16421:2014, IDT)

Вплив матеріалів на воду для споживання людиною. Посилення мікробного росту (EMG)

 
   
 
 
     
Не є офіційним виданням.
Офіційне видання розповсюджує національний орган стандартизації
(ДП «УкрНДНЦ» http://uas.gov.ua)

Contents

Foreword

Introduction

1 Scope

2 Normative references

3 Terms and definitions

4 Influence of materials on water for human consumption - Enhancement of microbial growth (EMG) - Method 1: Measured by Biomass Production Potential (BPP) measured by ATP

4.1 General

4.2 Principle

4.3 Apparatus

4.3.1 Requirements

4.3.2 Vessels, containers, stoppers and connectors and general laboratory apparatus

4.3.3 Cleaning

4.3.4 Gloves

4.3.5 Plates

4.3.6 Test containers

4.3.7 Test tubes

4.3.8 Incubator (or hot room)

4.3.9 Sterilisation ovens

4.3.10 Membrane filters

4.3.11 Autoclave

4.4 Reagents

4.4.1 Test water

4.4.2 Dilution water

4.4.3 Potassium dihydrogen orthophosphate solution

4.4.4 Potassium-nitrate solution

4.4.5 Sodium acetate solution

4.4.6 Inoculum

4.4.7 Specifications for controls

4.5 Test samples and controls

4.5.1 Sampling, transport and storage of test samples

4.5.2 Test piece preparation

4.6 Pre-treatment of test pieces

4.6.1 General

4.6.2 Flushing

4.6.3 Stagnation

4.6.4 Prewashing

4.7 Test procedure

4.7.1 Overview

4.7.2 ATP contamination

4.7.3 Preparation of the test container

4.7.4 Test pieces

4.7.5 Incubation

4.7.6 Positive and negative controls

4.7.7 Changing of test water

4.7.8 Measurement of ATP concentrations

4.8 Calculation of test results

4.8.1 Biomass Production (BP)

4.9 Reproducibility and repeatability

4.10 Test report

4.10.1 General

4.10.2 Material/Product information

4.10.3 Information on the test procedure

4.10.4 Test results

5 Influence of materials on water for human consumption - Enhancement of microbial growth (EMG) - Method 2: Measured by biofilm volume

5.1 General

5.2 Principle

5.3 Basic requirements

5.4 Apparatus and reagents

5.4.1 Disinfectant

5.4.2 Test water

5.4.3 Specifications for controls

5.4.4 Test container and test module for pipes and hoses

5.4.5 Scraper

5.4.6 Centrifuge

5.4.7 Glassware for centrifugation

5.4.8 Flowmeter

5.5 Test samples and test pieces

5.5.1 Sampling, transport and storage

5.5.2 Preparation of test pieces

5.6 Test procedure

5.6.1 General

5.6.2 Test procedure in test container

5.6.3 Test procedure in test module for pipes and hoses

5.6.4 Preparation of the test container or test module for pipes and hoses

5.6.5 Pre-treatment of test pieces

5.6.6 Removal of test pieces

5.6.7 Examination of test pieces

5.6.8 Re-exposure after harvesting

5.6.9 Centrifugation and determination of biofilm

5.6.10 Examination of biofilm

5.6.11 Test periods

5.7 Calculation of test results

5.8 Evaluation of satisfactory test performance

5.9 Test report

5.9.1 General

5.9.2 Material/Product information

5.9.3 Information on the test procedure

5.9.4 Test results

6 Influence of materials on water for human consumption - Enhancement of microbial growth (EMG) - Method 3: Measured by mean dissolved oxygen depletion

6.1 General

6.2 Principle

6.3 Test premises

6.3.1 General

6.3.2 Safety

6.4 Apparatus and reagents

6.4.1 General

6.4.2 Inoculum water

6.4.3 Test water

6.4.4 Test containers

6.4.5 Dissolved oxygen meter

6.4.6 30 °C Incubator/chamber

6.4.7 Polyethylene bags

6.4.8 Glass plates

6.4.9 Brass couplings

6.4.10 Special glass plates

6.5 Preparation of test pieces

6.5.1 General

6.5.2 Nature of test pieces

6.6 Test piece and test container size

6.6.1 General

6.6.2 Test sample storage

6.6.3 Special requirements

6.6.4 Specifications for controls

6.6.5 Test water control

6.7 Test procedures

6.7.1 Preparation of the test container

6.7.2 Incubation

6.7.3 Changing the test water

6.7.4 Measurement of dissolved oxygen

6.8 Calculation of results

6.8.1 Calculation of the mean dissolved oxygen difference (MDOD)

6.8.2 Validation of results

6.8.3 Repeatability and reproducibility

6.9 Test report

6.9.1 General

6.9.2 Material/Product information

6.9.3 Information on the test procedure

6.9.4 Results

Annex A (normative) Method 1: Measured by Biomass Production Potential (BPP) measured by ATP - Assessment of the suitability of test water for its use in the BPP test (biological stability and the need for addition of trace elements)

Annex B (normative) Method 1: Measured by Biomass Production Potential (BPP) measured by ATP - Protocol of ATP analysis

B.1 Introduction

B.2 Apparatus and glassware

B.3 Reagents and auxiliary substances

B.3.1 General

B.3.2 Sterile water

B.3.3 ATP solutions for standard dilutions and calibration curves

B.3.4 Luciferin/luciferase (enzyme) solution

B.3.5 ATP extraction reagent

B.4 Sampling

B.5 Procedure

B.5.1 General

B.5.2 Blank water

B.5.3 Control dilutions

B.5.4 Samples

B.6 Expression of results

B.6.1 Water samples

B.6.2 Material samples

B.7 Test record

B.8 Quality control of calibration curves

Annex C (informative) Method 1: Measured by Biomass Production Potential (BPP) measured by ATP - ATP and its measurement

Annex D (informative) Method 1: Measured by Biomass Production Potential (BPP) measured by ATP - Evaluating procedures for removal of attached biomass

D.1 Introduction

D.2 Procedures for removal of Attached Biomass

D.2.1 General

D.2.2 Swabbing

D.2.3 Low energy sonication (LES)

D.2.4 High energy sonication (HES)

D.3 Effectiveness of removal procedure

Annex E (informative) Method 1: Measured by Biomass Production Potential (BPP) measured by ATP

Annex F (informative) Method 2: Measured by biofilm volume

Annex G (informative) Method 2: Measured by biofilm volume

Annex H (informative) Method 3: Measured by mean dissolved oxygen depletion

Bibliography